| |
| DETAILED VIEW |
| VDRC ID |
111003 |
| Library |
KK Stocks (KK)
|
| Construct ID |
102930 |
| CG Number (r6.01) |
CG4236
|
| FlyBase gene number |
FBgn0086202
FBgn0263979
FBgn0015610
FBgn0263347
FBgn0025477
FBgn0016688
|
| Synonyms |
154659_at
55
caf1
caf-1
Caf1
Caf-1
CAF1
CAF-1
CAF1-55
caf1 p55
Caf1p55
Caf1/p55
CAF1p55
CAF1-p55
chromatin assembly factor 1
Chromatin Assembly Factor 1
Chromatin Assembly Factor-1
Chromatin assembly factor 1 subunit
Chromatin associated factor-1 subunit
dCAF1
d-CAF1
dCAF-1
dCAF-1 p55
dCAF-1-p55
dCAF1-p55
dNURF
MSI1/RbAp48/CAC3/LIN-53
Nucleosome remodeling factor
Nucleosome remodeling factor - 55kD
Nurf
NURF
Nurf55
Nurf 55
Nurf-55
NURF55
NURF 55
NURF-55
Nurf55/CAF1
p55
P55
p55CAF1
p55 CAF1
p55/NURF-55
RbAp48
S(ls)3
|
| Hairpin Length |
436 |
| ON Targets |
1
|
| OFF Targets |
0
|
| s19 |
1 |
| CAN Repeats |
4 |
| Viability |
lethal |
| Inserted Chromosome |
2 |
| Insertion @ 40D: |
No |
| Insertion @ 30B: |
Yes |
| |
|
| Left Primer PCR1 |
tggcgcccctagatgGCTTGGCATCTGCTACATGA
|
| Right Primer PCR1 |
taggtctagccccgcCGCTAATCTTGGCAGTGTGA
|
| Left Primer PCR2 |
GCGCGAATTCtggcgcccctagatg (ETU) |
| Right Primer PCR2 |
GCGCTCTAGAtaggtctagccccgc (XTS2) |
| Sequence Verification Primer |
ETU used as universal forward primer
|
| |
|
| Template |
genomic |
| Sequence |
GCTTGGCATC
TGCTACATGA
GTCTCTGTTC
GGATCGGTGG
CTGACGACCA
GAAGCTGATG
ATCTGGGACA
CGCGCAACAA
CAACACCTCG
AAGCCCTCGC
ATACAGTGGA
CGCTCACACA
GCGGAAGTCA
ATTGCTTGAG
CTTTAATCCC
TACTCGGAGT
TCATTCTGGC
CACGGGCTCC
GCTGACAAGA
CTGTAGCTCT
ATGGGATCTG
CGCAACCTCA
AGCTAAAACT
ACATTCCTTC
GAGTCGCACA
AGGACGAGAT
CTTCCAAGTG
CAGTGGTCGC
CGCATAACGA
GACTATTCTC
GCCTCCTCCG
GCACTGACCG
CCGCCTACAC
GTCTGGGATT
TGTCCAAAAT
CGGCGAGGAG
CAGAGTACGG
AGGATGCTGA
GGACGGACCA
CCCGAGCTGC
TCTTCATCCA
TGGCGGTCAC
ACTGCCAAGA
TTAGCG
|
| Comment |
KK RNAi line similar to VDRC ID 105838, but with insertion of UAS-RNAi construct in landing site at 30B only. No insertion at 40D. (See Vissers et al Nat. Comm. 2015. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. https://www.ncbi.nlm.nih.gov/pubmed/26758424). Stock donated by Kieran Harvey. |
| Publication |
Vissers et al Nat. Comm. 2016. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. |