| |
| DETAILED VIEW |
| VDRC ID |
111002 |
| Library |
KK Stocks (KK)
|
| Construct ID |
101055 |
| CG Number (r6.01) |
CG12072
|
| FlyBase gene number |
FBgn0013549
FBgn0011739
|
| Synonyms |
Dlats
dmLATS
l(3)100Aa
large tumor suppressor
large tumor supressor
lats
Lats
LATS
LATS1
Lats/Warts
lts
MENE (3R)-G
miracle-gro
wart
warts
Warts
warts/D-lats
Warts kinase
warts/large tumour suppressor
warts/lats
Warts/Lats
wts
Wts
wts/lats
Wts/Lats
WTS/LATS
|
| Hairpin Length |
379 |
| ON Targets |
1
|
| OFF Targets |
1
|
| s19 |
1 |
| CAN Repeats |
2 |
| Viability |
viable |
| Inserted Chromosome |
2 |
| Insertion @ 40D: |
No |
| Insertion @ 30B: |
Yes |
| |
|
| Left Primer PCR1 |
tggcgcccctagatgCACCCAGTTATTGTCGGCTT
|
| Right Primer PCR1 |
cgacgcccgctgataGTTCTTCATGGAGCAGCACA
|
| Left Primer PCR2 |
GCGCGAATTCtggcgcccctagatg (ETU) |
| Right Primer PCR2 |
GCGCTCTAGAcgacgcccgctgata (XTS1) |
| Sequence Verification Primer |
ETU used as universal forward primer
|
| |
|
| Template |
genomic |
| Sequence |
CACCCAGTTA
TTGTCGGCTT
CCGCGAGGAT
ATCCCTCTCG
GCCTTCACGT
GTGCCACCTG
ATTCCGCTTG
AGAACGTCCG
CTTTCCGCAG
GGTTTTCATC
GCATACAAAT
GGTTCGAGGT
ATCGATTTTG
CTCACCAGCG
TTACCTCGCC
AAATGCACCC
ACTCCAATGG
GCTTCAGTTT
GACGAACATG
CTCTTGTCCA
TCTTGGCGCG
CTTCAATCGA
ATGTAGTTGC
TCTCCTTTTG
GTTCAGCATT
TTCCTCATCT
CGATTTGGGT
CTGATCGGGC
AGTCCCACTT
TGTGCATCTC
CTTCTCCAGC
TGATTCTTGC
GATACGTGCG
CTGGCGATAC
GACTTGATCA
CGTTCTCTAT
GTGCTGCTCC
ATGAAGAAC
|
| Comment |
KK RNAi line similar to VDRC ID 106174, but with insertion of UAS-RNAi construct in landing site at 30B only. No insertion at 40D. (See Vissers et al Nat. Comm. 2015. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. https://www.ncbi.nlm.nih.gov/pubmed/26758424). Stock donated by Kieran Harvey. |
| Publication |
Vissers et al Nat. Comm. 2016. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. |