DETAILED VIEW
VDRC ID 111002
Library KK Stocks (KK)
Construct ID 101055
CG Number (r6.01) CG12072
FlyBase gene number FBgn0013549
FBgn0011739
Synonyms Dlats
dmLATS
l(3)100Aa
large tumor suppressor
large tumor supressor
lats
Lats
LATS
LATS1
Lats/Warts
lts
MENE (3R)-G
miracle-gro
wart
warts
Warts
warts/D-lats
Warts kinase
warts/large tumour suppressor
warts/lats
Warts/Lats
wts
Wts
wts/lats
Wts/Lats
WTS/LATS
Hairpin Length 379
ON Targets 1
OFF Targets 1
s19 1
CAN Repeats 2
Viability viable
Inserted Chromosome 2
Insertion @ 40D: No
Insertion @ 30B: Yes
   
Left Primer PCR1 tggcgcccctagatgCACCCAGTTATTGTCGGCTT
Right Primer PCR1 cgacgcccgctgataGTTCTTCATGGAGCAGCACA
Left Primer PCR2 GCGCGAATTCtggcgcccctagatg (ETU)
Right Primer PCR2 GCGCTCTAGAcgacgcccgctgata (XTS1)
Sequence Verification Primer ETU used as universal forward primer
   
Template genomic
Sequence CACCCAGTTA TTGTCGGCTT CCGCGAGGAT ATCCCTCTCG GCCTTCACGT GTGCCACCTG ATTCCGCTTG AGAACGTCCG CTTTCCGCAG GGTTTTCATC GCATACAAAT GGTTCGAGGT ATCGATTTTG CTCACCAGCG TTACCTCGCC AAATGCACCC ACTCCAATGG GCTTCAGTTT GACGAACATG CTCTTGTCCA TCTTGGCGCG CTTCAATCGA ATGTAGTTGC TCTCCTTTTG GTTCAGCATT TTCCTCATCT CGATTTGGGT CTGATCGGGC AGTCCCACTT TGTGCATCTC CTTCTCCAGC TGATTCTTGC GATACGTGCG CTGGCGATAC GACTTGATCA CGTTCTCTAT GTGCTGCTCC ATGAAGAAC
Comment KK RNAi line similar to VDRC ID 106174, but with insertion of UAS-RNAi construct in landing site at 30B only. No insertion at 40D. (See Vissers et al Nat. Comm. 2015. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. https://www.ncbi.nlm.nih.gov/pubmed/26758424). Stock donated by Kieran Harvey.
Publication Vissers et al Nat. Comm. 2016. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth.