| |
| DETAILED VIEW |
| VDRC ID |
111001 |
| Library |
KK Stocks (KK)
|
| Construct ID |
109756 |
| CG Number (r6.01) |
CG4005
|
| FlyBase gene number |
FBgn0034970
|
| Synonyms |
yap
YAP
yki
Yki
YKI
yorkie
Yorkie
|
| Hairpin Length |
629 |
| ON Targets |
1
|
| OFF Targets |
2
|
| s19 |
1 |
| CAN Repeats |
3 |
| Viability |
viable |
| Inserted Chromosome |
2 |
| Insertion @ 40D: |
No |
| Insertion @ 30B: |
Yes |
| |
|
| Left Primer PCR1 |
tggcgcccctagatgAACTGAATGGGGCTGATGAC
|
| Right Primer PCR1 |
tagcctccctagcgcCCGATCAAGTCCAACAACCT
|
| Left Primer PCR2 |
GCGCGAATTCtggcgcccctagatg (ETU) |
| Right Primer PCR2 |
GCGCTCTAGAtagcctccctagcgc (XTS4) |
| Sequence Verification Primer |
ETU used as universal forward primer
|
| |
|
| Template |
genomic |
| Sequence |
AACTGAATGG
GGCTGATGAC
GTCGTAGGAG
CGCTGCTTCT
TGTGCACTGT
CTGCATGGAC
ATGGGAATGT
CCTGCGACAT
GCAGGTGTTC
ATGGCGTCCA
GATCAATGGC
CGAGCTGGCC
GGGGCGCCGC
TGGGGAACTC
TTGGGCACTG
TTCTCTGGGA
AAGTGGGCCC
AGCGGGCTGC
TGTTGGCTGC
TCGGATTGGC
ATTCGGATTG
TTGGCTGCTG
CTGCGTCGCT
CCGGGCGCGC
ACATTGTAGT
TCTGCTGCAG
CGAGGCGGGG
CTGCTGCGGG
CTCGGGAGTG
ATGTATCGCC
AGGCGGCTGT
GCTGGGGAGA
CGGCTGAATC
TGGAGCTGGG
GGATGGCGGC
GATGGGCGAC
TGGCCATCTG
GCTGCTGGAC
GATGGACGCC
TTGTTCCCGA
TGTTGATGCT
CGACTGGGAG
CCCGCGTCGT
AGGTGGAGTC
GGCGCTGTTG
GCCCGCGAGT
GCGAGGGCGC
CGGGGGCGTG
AAGAAGGAGT
TGGGCAGCTT
CCGCATGCGC
AGGGGCAGCT
GCAGCGGGCG
CTTGGCGTCA
CCCGGATTCA
GGACGCTGTC
GAATAGCGCC
TGCAGGTTGT
CGTCCGTGTC
CTGGTTGACC
CGCACCACCA
GGTTGTTGGA
CTTGATCGG
|
| Comment |
KK RNAi line similar to VDRC ID 104523, but with insertion of UAS-RNAi construct in landing site at 30B only. No insertion at 40D. (See Vissers et al Nat. Comm. 2015. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. https://www.ncbi.nlm.nih.gov/pubmed/26758424). Stock donated by Kieran Harvey. |
| Publication |
Vissers et al Nat. Comm. 2016. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. |