| |
| DETAILED VIEW |
| VDRC ID |
111000 |
| Library |
KK Stocks (KK)
|
| Construct ID |
100936 |
| CG Number (r6.01) |
CG8208
|
| FlyBase gene number |
FBgn0027950
FBgn0029156
|
| Synonyms |
dMbD2/3
dMBD2/3
dMBD-like
Dm Mbd2/3
DmMbd2/3
MBD2-3
MBD2/3
MBD3
MBD-like
methyl-CpG-binding-domain-like-protein
|
| Hairpin Length |
384 |
| ON Targets |
1
|
| OFF Targets |
0
|
| s19 |
1 |
| CAN Repeats |
0 |
| Viability |
viable |
| Inserted Chromosome |
2 |
| Insertion @ 40D: |
No |
| Insertion @ 30B: |
Yes |
| |
|
| Left Primer PCR1 |
tggcgcccctagatgGACTGCCTCGATCTTCAAGC
|
| Right Primer PCR1 |
taggtctagccccgcCGTATGTCGTCCTCCGAAAT
|
| Left Primer PCR2 |
GCGCGAATTCtggcgcccctagatg (ETU) |
| Right Primer PCR2 |
GCGCTCTAGAtaggtctagccccgc (XTS2) |
| Sequence Verification Primer |
ETU used as universal forward primer
|
| |
|
| Template |
genomic |
| Sequence |
GACTGCCTCG
ATCTTCAAGC
AACCGGTGAC
GGTGATTCGC
AACCATAAAC
AGGATCCCGC
CAAAGCGAAG
AATGAACCCA
AGCATGGCAC
TCGGGAGAAG
CCGAAACAGC
TGTTCTGGGA
GAAGCGACTG
GAACGACTAC
GCGCCTGCCA
CGACAGTGGC
GAGGAGCTAG
ACGACATCTC
ACTGCCCAAG
ACCATACGCA
CCGTTGGCCC
CAATGTCAAC
GAACAGACCG
TCCTCCAGTC
GGTGGCCACG
GCCCTGCATA
TGCTCAACGC
CGGCGTACAC
GGCCAGAGTT
CTACGAAGGC
TGATTTGACC
AAGAATGCCA
TGGCGTTTAT
GAATCCTGAA
CAACCGCTAA
TGCACGCGGT
GATCATTTCG
GAGGACGACA
TACG
|
| Comment |
KK RNAi line similar to VDRC ID 107151, but with insertion of UAS-RNAi construct in landing site at 30B only. No insertion at 40D. (See Vissers et al Nat. Comm. 2015. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. https://www.ncbi.nlm.nih.gov/pubmed/26758424). Stock donated by Kieran Harvey. |
| Publication |
Vissers et al Nat. Comm. 2016. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. |