DETAILED VIEW
VDRC ID 111000
Library KK Stocks (KK)
Construct ID 100936
CG Number (r6.01) CG8208
FlyBase gene number FBgn0027950
FBgn0029156
Synonyms dMbD2/3
dMBD2/3
dMBD-like
Dm Mbd2/3
DmMbd2/3
MBD2-3
MBD2/3
MBD3
MBD-like
methyl-CpG-binding-domain-like-protein
Hairpin Length 384
ON Targets 1
OFF Targets 0
s19 1
CAN Repeats 0
Viability viable
Inserted Chromosome 2
Insertion @ 40D: No
Insertion @ 30B: Yes
   
Left Primer PCR1 tggcgcccctagatgGACTGCCTCGATCTTCAAGC
Right Primer PCR1 taggtctagccccgcCGTATGTCGTCCTCCGAAAT
Left Primer PCR2 GCGCGAATTCtggcgcccctagatg (ETU)
Right Primer PCR2 GCGCTCTAGAtaggtctagccccgc (XTS2)
Sequence Verification Primer ETU used as universal forward primer
   
Template genomic
Sequence GACTGCCTCG ATCTTCAAGC AACCGGTGAC GGTGATTCGC AACCATAAAC AGGATCCCGC CAAAGCGAAG AATGAACCCA AGCATGGCAC TCGGGAGAAG CCGAAACAGC TGTTCTGGGA GAAGCGACTG GAACGACTAC GCGCCTGCCA CGACAGTGGC GAGGAGCTAG ACGACATCTC ACTGCCCAAG ACCATACGCA CCGTTGGCCC CAATGTCAAC GAACAGACCG TCCTCCAGTC GGTGGCCACG GCCCTGCATA TGCTCAACGC CGGCGTACAC GGCCAGAGTT CTACGAAGGC TGATTTGACC AAGAATGCCA TGGCGTTTAT GAATCCTGAA CAACCGCTAA TGCACGCGGT GATCATTTCG GAGGACGACA TACG
Comment KK RNAi line similar to VDRC ID 107151, but with insertion of UAS-RNAi construct in landing site at 30B only. No insertion at 40D. (See Vissers et al Nat. Comm. 2015. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth. https://www.ncbi.nlm.nih.gov/pubmed/26758424). Stock donated by Kieran Harvey.
Publication Vissers et al Nat. Comm. 2016. A Drosophila RNAi library modulates Hippo pathway-dependent tissue growth.