DETAILED VIEW
VDRC ID 107092
Library KK Stocks (KK)
Construct ID 103240
CG Number (r6.01) CG10924
FlyBase gene number FBgn0003067
FBgn0034356
Synonyms
143299_at
CG17725
dPEPCK
Dromel_CG17725_FBtr0086701_pepck_mORF
pepck
Pepck
PEPCK
phosphoenol pyruvate carboxykinase
phosphoenolpyruvate carboxykinase
phosphoenolpyruvate carboxy kinase
Phosphoenolpyruvate carboxykinase
Phosphoenolpyruvate-carboxykinase
predicted phosphoenolpyruvate carboxykinase
Hairpin Length 401
ON Targets 1
OFF Targets 2
s19 0.98
CAN Repeats 2
Viability viable
Inserted Chromosome 2
Insertion @ 40D: No
Insertion @ 30B: Yes
   
Left Primer PCR1 tggcgcccctagatgCTGTCGCTTCCTCTCCATTC
Right Primer PCR1 taggtctagccccgcTGACATACATGGTGCGACCT
Left Primer PCR2 GCGCGAATTCtggcgcccctagatg (ETU)
Right Primer PCR2 GCGCTCTAGAtaggtctagccccgc (XTS2)
Sequence Verification Primer ETU used as universal forward primer
   
Template genomic
Sequence CTGTCGCTTC CTCTCCATTC AGTATGGAGA TGCAAAGATC CTCACCCCTG CCGTCAAACA GTACGTGGAA AAGTGCGTGG AGCTCTGCCA ACCGGAAAGG GTGCACATCT GTGATGGTAC TGAGGTCGAG AGCAAGTTGC TCCAGGGCAT CATGCTGAAG AAGGGAACCA TCATTCCTCT GCCGAAGTAC GAGAATTGCT GGCTGGCAAG AACCAATCCG GCGGATGTGG CTAGAGTGGA GGGAAAAACG TTCATCTCAA CGGATTCGAA GGAGCAGACA GTACCGGTTA CGGAGAAGGC CACACCGGGA ATGCTGGGTA ACTGGATAGC CGAAAAGGAC ATGCAGGCGG CGATCAAGGA AAGATTCCCC GGCTGCATGA AAGGTCGCAC CATGTATGTC A