| |
| DETAILED VIEW |
| VDRC ID |
106882 |
| Library |
KK Stocks (KK)
|
| Construct ID |
111404 |
| CG Number (r6.01) |
CG13565
|
| FlyBase gene number |
FBgn0034935
FBgn0062962
|
| Synonyms |
BcDNA:RH09340
|
| Hairpin Length |
348 |
| ON Targets |
1
|
| OFF Targets |
0
|
| s19 |
1 |
| CAN Repeats |
2 |
| Viability |
viable |
| Inserted Chromosome |
2 |
| Insertion @ 40D: |
No |
| Insertion @ 30B: |
Yes |
| |
|
| Left Primer PCR1 |
tggcgcccctagatgCCAGGATGCACCTGCTTTAT
|
| Right Primer PCR1 |
tagcctccctagcgcAACGGTCGTCTGCTACGAGT
|
| Left Primer PCR2 |
GCGCGAATTCtggcgcccctagatg (ETU) |
| Right Primer PCR2 |
GCGCTCTAGAtagcctccctagcgc (XTS4) |
| Sequence Verification Primer |
ETU used as universal forward primer
|
| |
|
| Template |
genomic |
| Sequence |
CCAGGATGCA
CCTGCTTTAT
TTTCTATAGT
CCCTTGACAT
GAAGCATTTT
AGAGTATTTC
CGCTATCAGA
GTCCGTATCC
ATATCCATGG
GCATTTACAT
TCACGCGCTG
TCGAAGGTAT
CACTAGACGA
GCTGGTTGAG
GATGCTGAAG
CTGGCGGAGG
CCTTGTCGAT
CTCGTCGAAG
TTCCGCTTGA
GCTTACTGCT
CCAGGTGTCC
TTGTACAGGG
GGTCCATCCT
CACCAGGTAG
GGCACCAGGT
CCCGGTGATG
GCCAGACCCG
ATCTTGCCGG
CCGCGCACTG
CTTCACCGAG
TACTTCGTCT
TGAACTCCGA
CTGGCTGCAC
TCGTAGCAGA
CGACCGTT
|