DETAILED VIEW
VDRC ID 105993
Library KK Stocks (KK)
Construct ID 102326
CG Number (r6.01) CG31033
FlyBase gene number FBgn0039706
FBgn0039705
FBgn0040624
FBgn0051033
Synonyms Atg16
Autophagy-specific gene 16
CG15513
CG18040
CG18683
Hairpin Length 344
ON Targets 1
OFF Targets 0
s19 1
CAN Repeats 4
Viability viable
Inserted Chromosome 2
Insertion @ 40D: No
Insertion @ 30B: Yes
   
Left Primer PCR1 tggcgcccctagatgTGCTCGTATATGCAACCCAA
Right Primer PCR1 taggtctagccccgcTTGGAGTCCCAACAACAACA
Left Primer PCR2 GCGCGAATTCtggcgcccctagatg (ETU)
Right Primer PCR2 GCGCTCTAGAtaggtctagccccgc (XTS2)
Sequence Verification Primer ETU used as universal forward primer
   
Template genomic
Sequence TGCTCGTATA TGCAACCCAA TATGCGCATG TGTGTTTATT TTTTTTTACA ATAGGAAAAC ATGTACTTAG ACTAACACTA TATGAATTGA TACAATTTGC ATACTATATC CGATTCACGT ATAAATAAAA TAATCAAGTA AAGTTGTTAA AACGTCGACC GAATTTACTA TGTACAGCAG ATTGCAGCAC TGCTCTGACT TCTTAAGCGT TTCAGTTTCG GAATTGGATT GCATCCGACT GGCTCGAGTG ACGTGTATGG CAACATTAGG GCTATGACTC CGAGTAGATG GTGCAGCGCT TGTTCTTTCC CACGGAGGCC AGCATGTTGT TGTTGGGACT CCAA