| |
| DETAILED VIEW |
| VDRC ID |
101575 |
| Library |
KK Stocks (KK)
|
| Construct ID |
103883 |
| CG Number (r6.01) |
CG8602
|
| FlyBase gene number |
FBgn0062051
FBgn0035763
FBgn0062076
|
| Synonyms |
anon-WO0118547.349
anon-WO0118547.382
|
| Hairpin Length |
367 |
| ON Targets |
1
|
| OFF Targets |
0
|
| s19 |
1 |
| CAN Repeats |
4 |
| Viability |
lethal |
| Inserted Chromosome |
2 |
| Insertion @ 40D: |
Yes (Info) |
| Insertion @ 30B: |
|
| |
|
| Left Primer PCR1 |
tggcgcccctagatgTTGACACAAAAACATGCGGT
|
| Right Primer PCR1 |
cgacgcccgctgataAGCGCCGAGAATTATCAGAA
|
| Left Primer PCR2 |
GCGCGAATTCtggcgcccctagatg (ETU) |
| Right Primer PCR2 |
GCGCTCTAGAcgacgcccgctgata (XTS1) |
| Sequence Verification Primer |
ETU used as universal forward primer
|
| |
|
| Template |
genomic |
| Sequence |
TTGACACAAA
AACATGCGGT
AACTTTAACT
TTAATAATAA
ATTGATCTAA
AAATACATAG
CGTAAAATAG
CATATAAACC
GGCCTGATCG
GGCTCGAATC
GCATATCAAT
ACTGATTCAT
GGCTGATCAG
GGCGTATTGT
ATATCCCTAT
CGTCGGATGA
CAAACAATTG
CAGTTAGTAA
ATACCAATTT
GCGACTCGAG
ACGCACATCA
CACATCATCA
TCACATCGTG
GAACGGACCA
AGGCGCATTG
CCCGCAGAGA
GTAACTTTTA
TGTCTACACT
AAATAACCGA
TAATAATCCG
ATCTGCGGGC
GCCGTTCGCC
TTTGTATCCC
CCCCTTTCTA
TTCAAAGTTC
TGATAATTCT
CGGCGCT
|