DETAILED VIEW
VDRC ID 100600
Library KK Stocks (KK)
Construct ID 108414
CG Number (r6.01) CG5002
FlyBase gene number FBgn0034275
Synonyms
Hairpin Length 271
ON Targets 1
OFF Targets 0
s19 1
CAN Repeats 2
Viability viable
Inserted Chromosome 2
Insertion @ 40D: No
Insertion @ 30B: Yes
   
Left Primer PCR1 tggcgcccctagatgAGACATCAAATGGGGCAATC
Right Primer PCR1 tagcctccctagcgcTTCCAGGATCGAAATCAAGG
Left Primer PCR2 GCGCGAATTCtggcgcccctagatg (ETU)
Right Primer PCR2 GCGCTCTAGAtagcctccctagcgc (XTS4)
Sequence Verification Primer ETU used as universal forward primer
   
Template genomic
Sequence AGACATCAAA TGGGGCAATC GTATATTCTG GAAGTACCTT GGTCTCTCCC GCAACGCCCT GGCTGTGATC TTTGGCACCT TTTTGGCCTA CATCCTCAGT CGAGATGGGA ATCAACCCTT CCGTGTCACT GGAAACATTA CGGCCGGAGT ACCGCCCTTC CGGCTGCCGC CCTTTAGCAC CACCGTAGAT GGAGAGTACG TTTCCTTTGG CGAGATGATC AGCACTGTCG GCGCATCCTT GGGTTCCATC CCCTTGATTT CGATCCTGGA A