| |
| DETAILED VIEW |
| VDRC ID |
100600 |
| Library |
KK Stocks (KK)
|
| Construct ID |
108414 |
| CG Number (r6.01) |
CG5002
|
| FlyBase gene number |
FBgn0034275
|
| Synonyms |
|
| Hairpin Length |
271 |
| ON Targets |
1
|
| OFF Targets |
0
|
| s19 |
1 |
| CAN Repeats |
2 |
| Viability |
viable |
| Inserted Chromosome |
2 |
| Insertion @ 40D: |
No |
| Insertion @ 30B: |
Yes |
| |
|
| Left Primer PCR1 |
tggcgcccctagatgAGACATCAAATGGGGCAATC
|
| Right Primer PCR1 |
tagcctccctagcgcTTCCAGGATCGAAATCAAGG
|
| Left Primer PCR2 |
GCGCGAATTCtggcgcccctagatg (ETU) |
| Right Primer PCR2 |
GCGCTCTAGAtagcctccctagcgc (XTS4) |
| Sequence Verification Primer |
ETU used as universal forward primer
|
| |
|
| Template |
genomic |
| Sequence |
AGACATCAAA
TGGGGCAATC
GTATATTCTG
GAAGTACCTT
GGTCTCTCCC
GCAACGCCCT
GGCTGTGATC
TTTGGCACCT
TTTTGGCCTA
CATCCTCAGT
CGAGATGGGA
ATCAACCCTT
CCGTGTCACT
GGAAACATTA
CGGCCGGAGT
ACCGCCCTTC
CGGCTGCCGC
CCTTTAGCAC
CACCGTAGAT
GGAGAGTACG
TTTCCTTTGG
CGAGATGATC
AGCACTGTCG
GCGCATCCTT
GGGTTCCATC
CCCTTGATTT
CGATCCTGGA
A
|