DETAILED VIEW
VDRC ID 100564
Library KK Stocks (KK)
Construct ID 108150
CG Number (r6.01) CG2158
FlyBase gene number FBgn0033264
Synonyms Nuclear pore protein 50
Nucleoporin 50
Nucleoporin 50kD
Nup
nup50
Nup50
Hairpin Length 276
ON Targets 1
OFF Targets 0
s19 1
CAN Repeats 3
Viability viable
Inserted Chromosome 2
Insertion @ 40D: Yes (Info)
Insertion @ 30B:  
   
Left Primer PCR1 tggcgcccctagatgCTCCAAGCGGTGTAAGGTGT
Right Primer PCR1 tagcctccctagcgcGCTTCGTCACCGGTCTTTAC
Left Primer PCR2 GCGCGAATTCtggcgcccctagatg (ETU)
Right Primer PCR2 GCGCTCTAGAtagcctccctagcgc (XTS4)
Sequence Verification Primer ETU used as universal forward primer
   
Template genomic
Sequence CTCCAAGCGG TGTAAGGTGT TCATTAAGAA AGACAAGGAC TTTGGCGATC GTGGCGTGGG CACGTTGTAC TTGAAACCTG TCAAGGACTC CGAAAAGATC CAACTGTTAG TCCGCGCCGA CACCAATCTG GGCAACATCT TGGTCAACCT TATTCTTAGC AAGGGTATAC CTTGCCAGCG CATGGGCAAG AACAATGTGC TGATGGTCTG CGTGCCCACG CCAGAGGACT CCAAGGCCAC ATCGCTGTTG CTACGCGTAA AGACCGGTGA CGAAGC