DETAILED VIEW
VDRC ID 100421
Library KK Stocks (KK)
Construct ID 104274
CG Number (r6.01) CG5732
FlyBase gene number FBgn0038934
Synonyms
Dm1
DmCG5732
DmGLD2
Gld2
Hairpin Length 516
ON Targets 1
OFF Targets 0
s19 1
CAN Repeats 3
Viability viable
Inserted Chromosome 2
Insertion @ 40D: No
Insertion @ 30B: Yes
   
Left Primer PCR1 tggcgcccctagatgCCAGCTAGATTGGCGAGTTC
Right Primer PCR1 taggtctagccccgcCTGGCCACAAAGATGGTTTT
Left Primer PCR2 GCGCGAATTCtggcgcccctagatg (ETU)
Right Primer PCR2 GCGCTCTAGAtaggtctagccccgc (XTS2)
Sequence Verification Primer ETU used as universal forward primer
   
Template genomic
Sequence CCAGCTAGAT TGGCGAGTTC GTCCGATGGC ATTGACCGTG AAGCAGTGGG CCCAGTATCA CAACATTAAC AATGCCAAGA ACATGACCAT ATCGAGCTAC TCGCTCATGC TAATGGTCAT TCATTTCCTG CAGGTTGGAG CAAGTCCACC TGTGCTTCCA TGTCTGCACA ACTTATATCC GGAGAAGTTT GGTCTCCTGC AGCCCAACGA TTTCGGTTAT GTGGACATGA ACGAGGTGAT GGCTCCGTAT CAGTCGGATA ACAGCCAAAC TCTAGGTGAT TTGCTGTTGA GCTTCCTACA CTACTACAGT GTATTTGATT ACGGAAAGTA TGCCATCTCG ATACGGGTGG GTGGTGTCCT GCCCATAGAG GTGTGCCGGG CAGCCACTGC GCCCAAAAAC GATATACACC AGTGGAACGA GCTGTGCATC GAAGAGCCAT TCGATCAGAC GAATACAGCT CGATCGGTGT ATGATACGGA TACATTTGAA CGCATCAAAA CCATCTTTGT GGCCAG