GD lines can be on chromosome 1, 2 or 3. All KK lines are on chromosome 2. All lines on chromosomes 2 or 3 are balanced. Lines either homozygous lethal or sterile are kept over the balancers (CyO for the 2nd chromosome; TM3, Sb for the 3rd chromosome). Stocks that are viable have been made homozygous.
Sterile lines are kept over the balancers. In these stocks there are both homozygous (these flies are sterile) and heterozygous flies for the UAS-IR insertion (over the CyO or TM3). For your RNAi experiments please use flies over the balancers and screen the progeny against the balancer.
Lethal lines are kept over the balancers. In these lines all the flies are either over the CyO or TM3,Sb balancer depending on the insertion chromosome. In these stocks homozygous flies (no balancer) survive rarely.
Insertions on the 1st chromosome are not balanced, and we keep these strains by crossing the RNAi males to X-compound virgins. In this case only the males have red eyes, never the females. Since these virgins (C(1)DX / winscy, hs-hid) are rather weak, we suggest adding fresh virgins every time you flip the RNAi stock on fresh food to ensure survival of the line. Or even better, balance the line with the 1st chromosome balancer. You can get a stock of (C(1)DX / winscy, hs-hid) in the RNAi Toolkit Stocks section (Stock Number 60006).
The red eye color - marks the successful insertion of the UAS-IR in the genome. As long as you do not find a fly with white eyes, you can be sure that there is no contamination in the stock. The eye color can vary from dark red to very light red. The eye color can vary even within the same stock.
All the UAS-IR insertion sites for the GD library are mapped only to the chromosome (1, 2 or 3). We do not have data on specific location on the chromosome.
The phenotype (viable, lethal, sterile) referred to in our database for every line, is due to the insertion site of the UAS-IR, not due the induction with the Gal4.
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As of Jan 2017 the library comprises 16,763 transgenic Drosophila strains of the P-Element RNAi library (GD),
9,822 lines of the phiC31 (KK) RNAi library and 372 of the shRNA library, which together target 12,671 genes, or
91% of the Drosophila melanogaster protein-coding genome.
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For the GD library, you should use the isogenic host strain w1118, which we injected the library into (VDRC ID 60000). For the KK library, the best control is
the host line y,w[1118];P{attP,y[+],w[3`]} (VDRC ID 60100). For the shRNA library use the host strain (VDRC ID 60200) containing an empty pWALIUM20 vector
(no hairpin sequence) integrated into the attP40 landing site, created in the same way and with same gene genetic background as the shRNA lines. You can find
these lines in our library under 'Browse RNAi Toolkit Stocks'. Alternatively, we suggest using a VDRC RNAi line which is known NOT to function in the process or pathway being studied.
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We refer to the phenotype due to the insertion site on the chromosome.
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These are the stocks which have been used for creation of the GD, KK and shRNA libraries and also lines which are helpful for triggering RNAi and increasing its efficiency e.g. dicer2.
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Yes, you can find them by clicking the ’Browse RNAi Toolkit Stocks’ button on our main page. Since January, 2008 you can also obtain these stocks from the Bloomington Drosophila Stock Center.
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Temporarily unavailable stocks are probably sick and being treated in hospital.
Usually it takes 3-5 weeks until we can release them back and are ready to be ordered.
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Your GD lines are insertions on the X chromosome. These lines are not balanced and we keep them by crossing the RNAi males to the X compound virgins.
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Some lines have been intentionally culled to streamline the collection, and a few have been lost over time due to the stock being too sick.
The DNA construct is usually available for re-injection.
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Please contact us: office@vdrc.at.
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Yes. The transgenes carry the 3xP3-dsred marker (Ejsmont et al., 2009). This is an eyeless derived
promoter fragment resulting in dsRed expression in the developing eye and in the brain. This needs to be
taken into account when working with the developing or adult brain. The eyes should appear white under the
light microscope but red under fluorescence.
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No, our MTA relates to transgenic flies and DNA constructs, so this is not necessary to have a VDRC
login and sign our MTA for ordering Fly Extract. However, you must fill out the ‘VDRC Fly Extract
order form’ each time you place an order.
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Fly Extract can only be sent on dry ice to ensure it is in a frozen condition when it arrives.
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Due to the different temperature and legal documentation requirements, orders of fly stocks/DNA
constructs and Fly Extract will be treated as completely independent orders and cannot be sent in a
combined shipment.
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You should pay the invoice upon receipt by wire transfer. It is not possible to pay the invoice by
credit card or by cheque.
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The time taken to dispatch your order will depend on the size of the order. Orders with 300 items or less will
be prioritized and dispatched within 2 weeks. For orders with a total of more than 300 items it may take up to 6 weeks for
your order to be dispatched. Orders with more than 150 lines will be split and delivered in two or more
shipments.
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E.g. The shipment fees for 30-40 lines to the USA would be around € 60-70.
Please contact your local delivery service for detailed information about shipping costs and see the link on VDRC website.
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You will be refunded 50% of the total cost of your order to compensate for the slow delivery.
You will be notified by email and your credit card will be refunded automatically.
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Smaller orders are charged a premium for dispatch within 2 weeks and are therefore prioritized.
Larger orders will be completed after smaller orders have been dispatched and we are therefore unable to guarantee such a fast turnaround time.
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In Europe the package is delivered within 2 working days. Shipments outside of Europe usually take few days longer e.g. Japan 2-3 days, USA/Australia up to one week.
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If you notify us within one week we will resend the lines but you will have to pay the shipping costs.
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Some countries (e.g. Australia, China) require a valid permit for importing transgenic Drosophila.
In the USA, you need a “Letter of No Permit Required”.
Please check the following link for detailed information: https://bdsc.indiana.edu/information/permits/import_routine.html
If you are located in Australia, please apply at the AQUIS (Australian Quarantine and Inspection Service) for an import license
and send a copy of the confirmed permission to us.
For importing lines into China, we need a permission for which you can apply for at the ’Bureau of Animal and Plant Health Inspection and Quarantine’
and a valid CR Number ’Customs Registration Number’.
Some other countries may also require permissions for importing transgenic Drosophila.
Please check your local importing terms. Please double-check the validation of the import permission.
The VDRC will not replace lines which have been refused at the customs because of missing or expired importing documents.
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We have a long and good experience with these courier services and also most of the researchers have their own accounts set up with them.
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After we shipped the flies we will send you the tracking number by e-mail.
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No, VDRC will provide you with an e-mail notification and the tracking number of the package the day the lines are shipped, but we cannot phone the customs,
USDA or change the shipping address after the package has been sent off.
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Please enquire: office@vdrc.at.
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It takes around 2 working days upon arrival of your signed MTA.
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It could be due to the following reasons:
(1) MTA did not arrive.
(2) The signatures are not valid. The MTA needs to be signed by a legal representative from your institution.
(3) You forgot to write the VDRC user name on the MTA and we cannot identify you.
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Yes, please login into your account and click on the button Order History placed in the upper right corner on the main page.
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As long as you send all necessary documents (import permits, shipping labels,..) there is usually no problem.
Your order is still in progress and, depending on the size of your order, it may take up to six weeks until your order is ready to ship.
Please check the Order History on the VDRC website.
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Unfortunately we cannot modify an existing order. Within 24h you can cancel the order with full refund and place another order with additional (or fewer) lines.
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Unfortunately we cannot modify an existing order.
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Yes, you can upload a list of CG numbers or a list of transformant IDs on the main page in the ’file upload box’.
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Our website does not offer a shopping cart management, but we recommend storing the Transformant ID of the desired lines in a list and to upload the list at a later date.
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Yes, since October 2010 it is possible to order the DNA constructs which we used to create our GD library.
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Yes, we accept signed MTAs sent by e-mail. .
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Sorry, but we cannot place an order for you. You have to do it yourself using the VDRC website.
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Yes of course. After placing the order, please send us an email to notify us. As soon as your order is ready to ship, we will contact you and make an appointment for picking up the lines.
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We have a limited capacity for researchers to perform their screens using VDRC lines on-site. Please send an email to office@vdrc.at to enquire.
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Just put the flies on fly food with NIPAGIN (15% sol, 12ml per 1l liquid fly food) added.
This ensures that the next generation of flies is totally free of mold and does not contaminate your stocks.
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The ordering and payment procedure differs depending on the total number of items you wish to order.
Orders of greater than 300 lines are automatically blocked and you are prompted to email office@vdrc.at and
ask us for a quote before you can proceed.
For further details see https://stockcenter.vdrc.at/control/howtoorder.
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Our price system is very attractive for large orders.
Please see our payment conditions.
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For security reasons we have no possibility to get detailed information about user transactions from financial institutes.
Therefore we recommend you to try again later and if it does not work to check with your bank.
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Your credit card is activated with the ’Verified by Visa’ service which improves the security of online payment transactions.
Therefore you need a password to verify your credit card transactions. Please contact your bank.
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For orders up to 300 items we can only accept credit card payments.
For orders bigger than 300 lines, we can only accept purchase orders which will be paid for via wire transfer.
Please see under How to place a purchase order for details.
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The Value Added Tax, or VAT, in the European Union is a general, broadly based consumption tax assessed on the value added to goods and services.
Every European Institution needs a VAT number for tax purposes.
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Yes of course, you will get full refund for all the items we could not deliver.
You will be notified and your credit card will be automatically refunded.
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For your convenience, you can now download the invoice/credit note in your VDRC account. Please follow these steps:
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Sorry, payment for orders of more than 300 lines is only possible with a Purchase Order code by wire transfer.