RNAi Protocols

GD Protocols

• Genomic DNA extraction
• mRNA extraction
• 1st strand cDNA
• 96 well cloning of RNAi constructs
• 96 well DNA purification using 'SigmaSpin Post-Reaction Clean-Up Plates'
• 96 well DNA gel extraction using 'NucleoSpin Multi-96 Extract'
• Fly transformation
• Bacteria Transformation 
• Transgene Verification 
• pMF3   [MAP]  [PDF]  [TEXT] 

KK Protocols

• Landing site VIE-260B, insertion on the 2nd chromosome at position 22019296 (5` to CR33987)
• Bacteria Transformation 
• Transgene Verification 
• pKC26   [MAP]  [PDF]  [TEXT] 
• pKC43   [MAP]  [PDF]  [TEXT]