DETAILED VIEW
VDRC ID 107209
Library KK Stocks (KK)
Construct ID 101856
CG Number (r6.01) CG7010
FlyBase gene number FBgn0028325
FBgn0029721
Synonyms CG 7010
l (1) G0334
l(1)G0334
L(1)g0334
lethal (1) G0334
Pdh
pyruvate dehydrogenase
Pyruvate dehydrogenase
Hairpin Length 511
ON Targets 1
OFF Targets 1
s19 1
CAN Repeats 3
Viability viable
Inserted Chromosome 2
   
Left Primer PCR1 tggcgcccctagatgGGAAGTGAAGCTGACCAAGG
Right Primer PCR1 cgcatgtagcctgccGCAGACAAAGATCACAGGCA
Left Primer PCR2 GCGCGAATTCtggcgcccctagatg (ETU)
Right Primer PCR2 GCGCTCTAGAcgcatgtagcctgcc (XTS3)
Sequence Verification Primer ETU used as universal forward primer
   
Template genomic
Sequence GGAAGTGAAG CTGACCAAGG ATCAGGCCCT CAAGTACTAC ACACAGATGC AGACGATCCG TCGGTTGGAG ACGGCCGCCG GTAATCTGTA CAAGGAGAAG ATCATTCGCG GCTTCTGTCA TCTGTATTCC GGCCAGGAGG CATGCGCCGT TGGCATGAAG GCGGCGATGC GGGATGTGGA CAACATCATC TCGGCGTACC GTGTCCACGG ATGGACCTAC CTGATGGGCG TCTCACCCAG CGGCGTACTG GCTGAGCTCA CCGGTGTCCA GGGTGGCTGT GCCCGCGGCA AGGGTGGCTC CATGCACATG TACGCACCGA ACTTCTACGG AGGCAATGGC ATCGTGGGTG CTCAAGTTCC TTTGGGTGCC GGCGTTGGCT TGGCCTGCAA GTACAAGGGC AATGGCGGCA TGTGCCTGGC CCTCTACGGT GATGGTGCCG CCAATCAAGG ACAGGTGTTC GAGGCTTACA ACATGGCCTA CCTGTGGAAA CTGCCTGTGA TCTTTGTCTG C